By Plamen Demirev, Todd R. Sandrin
In the final region century, advances in mass spectrometry (MS) were on the vanguard of efforts to map advanced organic platforms together with the human metabolome, proteome, and microbiome. All of those advancements have allowed MS to turn into a well-established molecular point expertise for microorganism characterization. MS has validated its huge virtue as a fast, actual, and affordable strategy for microorganism identity, in comparison to traditional phenotypic strategies. within the final a number of years, functions of MS for microorganism characterization in examine, medical microbiology, counter-bioterrorism, nutrients defense, and environmental tracking were documented in hundreds of thousands of courses. Regulatory our bodies in Europe, the U.S., and in other places have licensed MS-based assays for infectious ailment diagnostics. As of mid-2015, greater than 3300 advertisement MS structures for microorganism identity were deployed world wide in hospitals and medical labs.
While past paintings has coated broader ways in utilizing MS to signify microorganisms on the species point or above, this booklet specializes in strain-level and subtyping functions. In twelve person chapters, innovators, leaders and practitioners within the box from all over the world have contributed to a entire review of present and next-generation ways for MS-based microbial characterization on the subspecies and pressure degrees. Chapters contain updated reference lists in addition to web-links to databases, instructed software program, and different necessary tools.
The emergence of recent, antibiotic-resistant traces of human or animal pathogens is of notable problem not just to the clinical and clinical groups, yet to most of the people besides. advancements of novel MS-based assays for fast id of traces of antibiotic-resistant microorganisms are reviewed within the e-book to boot. Microbiologists, bioanalytical scientists, infectious sickness experts, scientific laboratory and public overall healthiness practitioners in addition to researchers in universities, hospitals, executive labs, and the pharmaceutical and biotechnology industries will locate this booklet to be a well timed and invaluable source.
Read or Download Applications of Mass Spectrometry in Microbiology: From Strain Characterization to Rapid Screening for Antibiotic Resistance PDF
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Additional info for Applications of Mass Spectrometry in Microbiology: From Strain Characterization to Rapid Screening for Antibiotic Resistance
Following the data processing 2 Methods and Instrumentation in Mass Spectrometry for the Differentiation … 37 Fig. 8 Comparison of MALDI-MS profile (protein extraction; sinapinic acid matrix) with the LC-MS mass profile for Shigella sonnei. The x-axis represents the mass of the neutral proteins, M, and not the m/z of the [M + nH]n+ ions in the ESI- or MALDI-mass spectra. (Adapted from Everley et al. 9). 9) between the samples. These mass shifts most likely represent protein products of SNP containing genes that differentiate these two strains.
Orbitrap-based MS systems are predominantly used for bottom-up and middle-down proteomic measurements, (Cannon et al. 2010) where the peptides are fragmented in the linear ion trap and fragments mass analyzed in the orbitrap. In addition, orbitrap-MS systems are used for accurate mass determination of intact proteins (McFarland et al. 2014). These measurements are particularly useful for the detection and differentiation of closely related microorganisms since this methodology allows the detection of a wide range of proteins in the sample, beyond the detection of ribosomal proteins.
Cech NB, Enke CG. Practical implications of some recent studies in electrospray ionization fundamentals. Mass Spectrom Rev. 2001;20(6):362–87. Chang C-J, Lin J-H, Chang K-C, Lai M-J, Rohini R, Hu A. Diagnosis of Β-Lactam resistance in Acinetobacter baumannii using shotgun proteomics and LC-Nano-Electrospray ionization ion trap mass spectrometry. Anal Chem. 2013;85:2802–8. Chennamaneni NK, Kumar AB, Barcenas M, Spáčil Z, Scott CR, Tureček F, Gelb MH. Improved reagents for newborn screening of mucopolysaccharidosis Types I, II, and VI by tandem mass spectrometry.
Applications of Mass Spectrometry in Microbiology: From Strain Characterization to Rapid Screening for Antibiotic Resistance by Plamen Demirev, Todd R. Sandrin