Download e-book for iPad: Animals with Novel Genes by Norman Maclean

By Norman Maclean

ISBN-10: 0511574614

ISBN-13: 9780511574610

ISBN-10: 0521024722

ISBN-13: 9780521024723

ISBN-10: 0521432561

ISBN-13: 9780521432566

This publication is set transgenic animals--animals into which new genes were artificially brought. It has chapters via major specialists at the current state-of-play in regards to the program of the transgenic know-how to assorted animal teams. Scientists have carried out experiments on a good variety of animal varieties, starting from protozoan cells, via nematode worms and fruit flies to many greater vertebrates, and this e-book presents an in-depth, updated, accomplished and authoritative account of the paintings with all such animal forms. The most likely influence of transgenic animals at the way forward for agriculture and drugs is especially nice. those animals supply an unequalled experimental process for the research of gene rules, genetic elements of ailment, and gene remedy. one of many targets of the ebook is as a result to set transgenic animals within the context in their current and destiny contributions to technological know-how and medication.

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Black and Rai (1988) have analysed the genomic DNA from four species of mosquito, namely Anopheles quadrimaculatus, Culex pipiens, Aedes albopictus and Aedes triseriatus. More recently, Cockburn and Mitchell (1989) have shown that the genomes of anopheline mosquitoes are generally relatively small and exhibit the LPI pattern of repetitive sequences. 7 x 108 bp (Besansky & Powell 1992). This is in marked contrast to Aedes aegypti, which has the more complex SPI pattern (Warren & Crampton 1991). 3 x 108 bp), being five times the size of the Drosophila genome, or, to place this in context, one-third the size of the human genome.

The dominant selectable marker, along with any necessary controlling sequences, is also carried within the ITRs. Functional transposase is supplied in trans to this transformation vector by a 'helper element' which carries all of the transposase coding sequence but is rendered incapable of transposition itself by deletions to one ITR or both. This separation of 'vector' and 'helper' functions means that vector sequences can be integrated, generally as single copies, into the recipient genome, where they will remain stable, unless remobilized either by a further microinjection of 'helper' elements or purified P transposase (Kaufman & Rio 1991) or by the introduction of active P elements into the genome by way of a conventional cross.

Butlrick, R. , McNally, E. , Kaplan, M. L. & Leinwand, L. A. (1991). Hormonal modulation of a gene injected into rat heart in vivo. Proc. Natl. Acad. Sci. 88, 4138-42. McKnight, R. , Wall, R. J. & Hennighausen, L. (1992). Matrix-attachment regions can impart position-independent regulation of a tissue-specific gene in transgenic mice. Proc. Natl. Acad. Sci. 89, 6943-7. Miller, A. D. (1992). Human gene therapy comes of age. Nature 357, 455-60. Mulligan, R. C. (1993). The basic science of gene therapy.

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Animals with Novel Genes by Norman Maclean

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