Animal cell technology: from biopharmaceuticals to gene - download pdf or read online

By Leda R. Castilho

ISBN-10: 0203895169

ISBN-13: 9780203895160

ISBN-10: 041542304X

ISBN-13: 9780415423045

Creation to animal mobilephone know-how /, Animal cells: easy suggestions /, Cloning and expression of heterologous proteins in animal cells /, phone metabolism and its keep an eye on in tradition /, tradition media for animal cells /, Post-translational amendment of recombinant proteins /, Mechanisms of mobile proliferation and phone loss of life in animal telephone tradition in vitro /, Mathematical versions for development and product synthesis in animal telephone tradition /, Bioreactors for animal cells /, tracking and keep an eye on of phone cultures /, Animal phone separation /, Product purification approaches /, qc of biotechnological items /, Regulatory facets /, highbrow estate /, Recombinant healing proteins /, Monoclonal antibodies /, Viral vaccines: recommendations, rules, and bioprocesses /, Bioinsecticides /, cellphone cures and stem cells /, Gene treatment /; Paula Marques Alves, Manuel Jose Teixeira Carrondo, & Pedro Estilita Cruz --, Patricia Leo ... [et al.] --, Mariela Bollati-Fogolin & Marcelo A. Comini --, Paola Amable & Michael Butler --, Angela Maria Moraes, Ronaldo Zucatelli Mendonca, & Claudio Alberto Torres Suazo --, Michael Butler --, Maira Peixoto Pellegrini, Rodrigo Coelho Ventura Pinto, & Leda dos Reis Castilho --, Elisabeth F.P. Augusto, Manuel F. Barral, & Rosane A.M. Piccoli --, Ernesto Chico Veliz, Gryssell Rodriguez, & Alvio Figueredo Cardero --, Aldo Tonso --, Leda dos Reis Castilho & Ricardo de Andrade Medronho --, Angela Maria Moraes, Leda Castilho, & Sonia Maria Alves Bueno --, Marina Etcheverrigaray & Ricardo Kratje --, Maria Teresa Alves Rodrigues & Ana Maria Moro --, Ana Cristina Almeida Muller & Leila Costa Duarte Longa --, Maria Candida Maia Mellado & Leda dos Reis Castilho --, Wirla M.S.C. Tamashiro & Elisabeth F.P. Augusto --, Isabel Maria Vicente Guedes de Carvalho Mello ... [et al.] --, Marcia Regina da Silva Pedrini & Ronaldo Zucatelli Mendonca --, Hamilton da Silva Jr. & Radovan Borojevic --, Celio Lopes Silva ... [et al.]

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Hot Drugs (2004), Nat. Rev. , Suppl. Vol. 3. Hwang WS, Ryu YJ, Park JH, Park ES, Lee E, Koo, JM, Jeon HY, Lee BC, Kang SK, Kim SJ, Ahn C, Hwang JH, Park KY, Cibelli JB, Moon SY (2004), Evidence of a pluripotent human embryonic stem cell line derived from a cloned blastocyst, Science 303:1669–1674. Kohler G, Milstein C (1975), Continuous cultures of fused cells secreting antibody of predefined specificity, Nature 256:495–497. Laflamme MA, Murry CE (2005), Regenerating the heart, Nat. Biotechnol. 23: 845–856.

He considered three hypotheses: (i) in situ formation from the nerve sheath; (ii) preformed protoplasmic bridges; or (iii) as a result of the nerve cell growth itself. When Harrison demonstrated the validity of the third hypothesis, he also confirmed the cell as the primary developing unit of multicellular organisms. An early pioneer of cell culture was the French surgeon Alexis Carrel, who won the Nobel prize in Medicine in 1912 for his research at the Rockefeller Institute (Spier, 2000). Harrison was, above all, the inventor of analytical solutions, while Carrel, with his extensive clinical practice experience, sterility concerns, and capacity to develop appropriate culture media and culture flasks, created the change in the technological paradigm that led to the start up of animal cell technology.

In this process, termed exocytosis, the vesicle content is secreted into the outer environment. 6 Mitochondria Mitochondria are spherical or, more frequently, elongated bodies, consisting of two membrane units. The inner one is folded in the form of tubules or shelves, named cristae.

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Animal cell technology: from biopharmaceuticals to gene therapy by Leda R. Castilho

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