By Dr Desmond S. T. Nicholl
Des Nicholl offers a brand new, absolutely revised, and elevated variation of his renowned undergraduate-level textbook. The e-book keeps a number of the beneficial properties of the unique version and nonetheless bargains a concise technical advent to the topic of genetic engineering. it's divided into 3 major sections: easy molecular biology, tools of gene manipulation, and smooth functions of genetic engineering. purposes lined within the ebook comprise genomics, protein engineering, gene remedy, cloning, transgenic animals and crops and bioethics. An creation to Genetic Engineering is key analyzing for undergraduate scholars of biotechnology, genetics, molecular biology, and biochemistry.
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Additional info for An Introduction to Genetic Engineering
6), which codes for the enzymes responsible for lactose catabolism. Within the operon there are three genes that code for proteins (termed structural genes) and an upstream control region encompassing the promoter and a regulatory site called the operator. In this control region there is also a site which binds a complex of cyclic AMP and CRP (cyclic AMP receptor protein), which is important in positive regulation (stimulation) of transcription. Lying outside the operon itself is the repressor gene, which codes for a protein (the Lac repressor) that binds to the operator site and is responsible for negative control of the operon by blocking the binding of RNA polymerase.
Rapid methods for sequence analysis were developed in the late 1970s, and the technique is now used in laboratories worldwide. 36 Working with nucleic acids Fig. 6. Black-and-white photograph of an agarose gel, stained with ethidium bromide, under UV irradiation. The DNA samples show up as orange smears or as orange bands on a purple background. Individual bands (lane 1) indicate discrete fragments of DNA – in this case, the fragments are of phage DNA cut with the restriction enzyme HindIII. The sizes of the fragments (in kb) are indicated.
It is therefore important that reliable methods are available for isolating these components from cells. There are three basic requirements: (i) opening the cells in the sample to expose the nucleic acids for further processing, (ii) separation of the nucleic acids from other cell components, and (iii) recovery of the nucleic acid in puriﬁed form. A variety of techniques may be used, ranging from simple procedures with few steps, up to more complex puriﬁcations involving several diﬀerent stages.
An Introduction to Genetic Engineering by Dr Desmond S. T. Nicholl